GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Combretum Hispidum Laws Combretaceae Leaves

Phytoconstituents present in the ethanolic extract of Combretum hispidum leaves were explored by Gas Chromatography Mass Spectrometry analysis. The compounds were identified by the gas chromatography coupled with the mass spectrometry. The molecular weight and structure of the compounds of Combretum hispidum leaves were ascertained by interpretation of the spectrum of GC MS using the database of National Institute of Standard and Technology NIST . GC MS analysis of Combretum hispidum leaves revealed the presence of nineteen biological active compounds. The compounds are N Tosyl dl 3,4 dehydroprolylglycine, ethyl ester, 1 n Butoxy 2,2,3,3 tetramethylaziridine, 2 Butenoic acid, 3 methyl 4 tetrahydro 3,4 dihydroxy 5 3 2 hydroxy 1 methylpropyl oxiranyl methyl 2H pyran , Cobalt, octacarbonyl zinc di , 2Co Zn , 6 Dehydroxy 2,3,3,4,4,5,7 hepta O methylisoorientin, 2 naphthalenol, 3 5 3 nitrophenyl 1,3,4 oxadiazol 2 yl , L Proline, N 1 naphthoyl , dodecyl ester, 3,6 Dispirocyclohexyl 1,2,3,4,5,6,7,8 octahydro 1,8 acridinedione, Sarcosine, N 2 chloroethoxycarbonyl , heptadecyl ester, Cycloocta 1,2 b 4,3 b 5,6 b 8,7 b tetrakis 1 benzothiophene, Butanoic acid, 2 chloro 3 methyl , 4 5 heptyl 2 pyrimidinyl phenyl ester, Friedooleanan 1 one, 3,24 dihydroxy , 9 O Methyl 4,5 deoxymaytansino, Ditelluride, di 1 naphthalenyl, tert Butylstibinous acid thioanhydride, l Leucine, N methyl n pentadecafluorocarbonyl , octadecyl ester, 2,5 Dichloro N ethyl N phenyl benzamide, 2 Thiophenylacetic acid, 2,2,2 trifluoroethyl ester, Methyl 8 5 methoxycarbonyl methyl 2 furyl octanoate. It was concluded that the bioactive compounds support the use of C. hispidum leaves in the treatment of diseases like cancer, anaphylactic shock, renal failure, diabetes and hypertension. 

by O. V. Ikpeazu | I. E. Otuokere | K. K. Igwe "GC–MS Analysis of Bioactive Compounds Present in Ethanol Extract of Combretum Hispidum (Laws) (Combretaceae) Leaves" 

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-5 , August 2020, 

URL: https://www.ijtsrd.com/papers/ijtsrd31868.pdf 

Paper Url :https://www.ijtsrd.com/chemistry/biochemistry/31868/gc–ms-analysis-of-bioactive-compounds-present-in-ethanol-extract-of-combretum-hispidum-laws-combretaceae-leaves/o-v-ikpeazu

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Colorimetric Determination of Stability Constant of Trimethoprim Cu II Complex at Different Temperatures by Continuous Variation Method

 Trimethoprim is an antibiotic that is mainly used for the treatment of bladder infections. Continuous variation method was applied in the calculation of stability constant of trimethoprim Cu II complex depending on the theoretical explanation of the stoichiometry. The formation of Cu II complex with trimethoprim was studied colorimetrically at an absorption maximum of 480 nm at 25, 30, 35 and 40 oC. The data showed that Cu II and trimethoprim combine in the molar ratio of 1 1 at pH 7.4 with ionic strength maintained using 0.1M KNO3. Calculated stability constants values were 6.00 x 104, 3.80 x 104, 2.61 x 104 and 1.89 x 104 at 25, 30, 35 and 40 oC respectively. Calculated GO for the complex were 2.73 x 104, 2.66 x 104, 2.60 x 104 and 2.56 x 104 at 25, 30, 35 and 40 oC respectively. The stoichiometry, stability constant and Gibbs free energy results suggested that trimethoprim used in the study is a potent chelating agent and can be an efficient antidote in the therapy of Cu II overload or poisoning. 

by O. V. Ikpeazu | I. E. Otuokere | K. K. Igwe "Colorimetric Determination of Stability Constant of Trimethoprim-Cu(II) Complex at Different Temperatures by Continuous Variation Method" 

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-5 , August 2020, 

URL: https://www.ijtsrd.com/papers/ijtsrd31849.pdf 

Paper Url :https://www.ijtsrd.com/chemistry/biochemistry/31849/colorimetric-determination-of-stability-constant-of-trimethoprimcuii-complex-at-different-temperatures-by-continuous-variation-method/o-v-ikpeazu

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